Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 4. Effect of yak milk on immunohistochemistry of kidney in mice. (A) Images of mouse kidney tissue cross sections immunostained for TRPV5 and calbindin-D28k (scale bar = 100 μm). (B) Relative mean density of TRPV5 in mouse kidney. (C) Relative mean density of calbindin-D28k in mouse kidney. Mean density = (integrated optical density [IOD] sum)/area. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Immunohistochemistry

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 5. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k in mouse kidney. (A) Western blot showing the expression of TRPV5 and calbindin-D28k in mouse kidney. (B) Quantification of TRPV5 expression from the Western blots. (C) Quantification of calbindin-D28k expression from the Western blots. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with dif- ferent lowercase letters (a–c) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing, Western Blot

Journal: Journal of dairy science

Article Title: Yak milk promotes renal calcium reabsorption in mice with osteoporosis via the regulation of TRPV5.

doi: 10.3168/jds.2022-23218

Figure Lengend Snippet: Figure 6. Effects of yak milk on the expression of calcium transporters TRPV5 and calbindin-D28k mRNA in mouse kidney. (A) Relative expression of TRPV5 mRNA in mouse kidney. (B) Relative expression of calbindin-D28k mRNA in mouse kidney. L-YM = low-dose yak milk group, 1,000 mg/kg per day; H-YM = high-dose yak milk group, 2,000 mg/kg per day; ALN = alendronate sodium group, 1 mg/kg per day. Data are expressed as mean ± SD. Means with different lowercase letters (a–d) in a column are significantly different (P < 0.05).

Article Snippet: The anti-TRPV5 antibody (M03218), anticalbindinD28k antibody (PB9045) and hypersensitive chemiluminescence kit (AR1174) were procured from BOSTER Biological Technology Co. Ltd.

Techniques: Expressing

Journal: Journal of Human Genetics

Article Title: Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family

doi: 10.1038/s10038-019-0651-z

Figure Lengend Snippet: Compound heterozygous variants in MYH11 in a family with MMIHS. a Prenatal ultrasonography image at 13 weeks of gestation for the index fetus demonstrated a distended bladder (2.56 cm × 2.32 cm). b Prenatal ultrasonography image at 17 weeks shows a progressive distention of the bladder (9.5 cm × 7.16 cm) in the index fetus. c Sanger sequencing validates the exome sequencing variant of c.2051 G > A (p.R684H) in MYH11 (NM_001040114). d Sanger sequencing validates the exome sequencing variant of c.3540_3541delinsTT (p.(E1180D, Q1181Ter)) in MYH11 (NM_001040114). e Protein expression of MYH11 in the control (Ctrl) and proband umbilical cord tissues. Arrows point to the band location for protein MYH11

Article Snippet: Anti-MYH11 antibody (1:50, Monoclonal rabbit IgG, BM5659, Boster Biological Technology, China) was used as a primary antibody.

Techniques: Sequencing, Variant Assay, Expressing, Control

Journal: Journal of Human Genetics

Article Title: Compound heterozygous variants in MYH11 underlie autosomal recessive megacystis-microcolon-intestinal hypoperistalsis syndrome in a Chinese family

doi: 10.1038/s10038-019-0651-z

Figure Lengend Snippet: Summary of clinical and molecular findings of four genes involved in autosomal recessive MMIHS

Article Snippet: Anti-MYH11 antibody (1:50, Monoclonal rabbit IgG, BM5659, Boster Biological Technology, China) was used as a primary antibody.

Techniques: Sequencing

Journal: Human Molecular Genetics

Article Title: Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia

doi: 10.1093/hmg/ddy439

Figure Lengend Snippet: Antibodies for immunohistochemistry

Article Snippet: After incubation with primary antibodies, slides were washed three times in TBST (TBS buffer + 0.1% tween 20) for 5 min, incubated with secondary antibodies at RT for 1 h and then washed three times in TBST before being mounted with Prolong Gold Anti-fade Reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen) ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Company (Catalog number) Dilution FGF10 Antibodies-online (ABIN360398) 1:30 (human) FGFR2 (BEK) Santa Cruz (sc-122) 1:300 (human) SFTPC Seven Hills (WRAB-9337) 1:100 (human) PECAM/CD31 BD Pharmingen (550274) 1:100 (mouse and human) α -SMA Sigma-Aldrich (A2547) 1:600 (mouse) 1:400 (human) von-Willebrand-factor (VWF) Dako (A0082) 1:1200(mouse) MYL6b Merck (AB3381) 1:100 (mouse) 1:200 (human) Phospho MYL6b BosterBio (A14059) 1:600 (mouse) 1:200 (human) MYL9 Abcam (ab64161) 1:200 (mouse and human) Phospho MYL9 Abcam (ab2480) 1:100 (mouse and human) Open in a separate window Antibodies for immunohistochemistry FGFR2 and FGF10 staining Immunohistochemistry for FGFR2 and FGF10 was performed on lung tissue sections from patients with BPD.

Techniques:

Journal: Proteome Science

Article Title: Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus

doi: 10.1186/1477-5956-9-11

Figure Lengend Snippet: List of differentially expressed protein spots in tracheal tissues identified by MALDI-TOF-TOF MS and MS/MS analysis

Article Snippet: 2 , gi|212347 , Myosin light chain 1, skeletal muscle isoform [ Gallus gallus ] , 19.5/4.96 , 192 , 53 , 0.6040 ± 0.0519 , 0.3576 ± 0.0656 , 0.007 , 0.59 , Motor activity. Calcium ion binding. , .

Techniques: Infection, Activity Assay, Binding Assay, Membrane, Ubiquitin Proteomics, Protein Binding, Activation Assay, Transduction